BCHM 444 Spring 2008 Biochemical Methods in Molecular Biology

Martin Teintze

Office: Room 155, Chemistry & Biochemistry Building; Lab: Room 158

Phone: 994-5390

E-mail: mteintze@montana.edu

Syllabus and Lab Schedule

Papers on Alkaline Phosphatase

Lab Protocols

 

Mutation proposals

Presentation Assignments

Library Search Tutorial

Literature Search Tutorial

Final Paper Instructions

Sequences

 

Data

 

Calculations: Sample spreadsheet and explanation

Primer Design websites: IDToligo calc (seems to work better with IE), MIT primer3 (picks primers for amplification, not mutagenesis), Bioinformatics.net (has links to variety of primer design sites), PrimerX (for mutagenesis)

 

Sequence manipulation websites:

http://ca.expasy.org/tools/dna.html  translates to protein

http://www.ch.embnet.org/software/LALIGN_form.html  aligns 2 sequences

http://www.ualberta.ca/~stothard/javascript/rev_comp.html  makes reverse complements

NCBI databases of protein structures and DNA sequences: select database from menu bar across top

Protein Structural Genomics Database: http://kb.psi-structuralgenomics.org/KB