Metabolite levels are closely related to cellular phenotypes. Further, in cells, the number of metabolites is much lower than the number of genes or proteins. While metabolic changes are regulated by gene expression, they are widely influenced by environmental factors, such as stress. We thus anticipate that this research will enhance our ability to recognize “lead†diagnostic markers of cellular responses to the stress of pathogen exposure. Our collaborative research projects consist currently of: (1) metabolic profiling of microbial biofilms involved in abnormal healing of chronic wounds; (2) profiling of metabolites in Sulfolobus strains that are resistant or very susceptible to crenarchaeal viral infections compared to wild-type organisms; (3) metabolite profiling of E. coli strains engineered via gene knock-outs followed by adaptive evolution; (4) “From genomes to metabolomes: Understanding mechanisms of symbiosis and cell-cell signaling using the archaeal system Ignicoccus-Nanoarchaeum“ The goal of this project is to characterize mechanisms of cellular recognition and signaling, gene expression and metabolic processes associated with symbiosis in the archaeal system Ignicoccus hospitalis and Nanoarcheum equitans. This project aims to provide fundamental insights into the molecular mechanisms of host-microbe interactions.

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Spectroscopy, Protein Chemistry, NMR, Chemical Biology, Biophysical, Biochemistry, Analytical