MSU Nuclear Magnetic Resonance Core Facility
NMR RESOURCES AT MONTANA STATE UNIVERSITY
The MSU Nuclear Magnetic Resonance (NMR) Facility is a campus-wide Core Resource Facility which is financially supported by user fees and funds from the Office of the Vice President of Research and Economics Development and the Dean of the College of Letters and Science. The NMR facility is located in the Chemistry and Biochemistry building (room 18) at MSU and provides state-of-the-art solution NMR spectroscopy instrumentation, education, methodology and training services. Access to these resources and services is granted to academic researchers both at MSU and outside universities as well as to off-campus users from industry.
The facility presently operates three high field solution NMR spectrometers with specialized NMR probes and high-throughput capabilities. The facility strives to offer the latest in NMR capabilities and advancements implemented for small molecule structure elucidation, biomolecular–protein structure determination, quantitative/purity analysis, impurity/unknown determination and metabolomics analysis. The facility is an open-access resource with 24/7 accessibility for MSU scientists and faculty who require rapid sample analysis and time-sensitive NMR experiments. The NMR facility interacts closely with the Mass Spectrometry Proteomics and Metabolomics Core Facility to offer a complete and integrated approach to solving complex chemical and biochemical research problems quickly, including metabolomics research.
Most recently, Dr. Copié (recently appointed Director of MSU’s NMR Center by MSU’s VPR) and colleagues applied to the NSF-MRI (awarded) and Murdock Charitable Trust Foundation (awarded) grant programs to acquire a ~ $1M state-of-the-art integrated LC-SPE-NMR-MS instrument, involving the acquisition of a new ProdigyTM cryoprobe-equipped (e.g. X-nucleus detection optimized) 500 MHz NMR system, which will be coupled to a mass spectrometer instrument for simultaneous MS and NMR analysis (i.e. MS and NMR analysis of the same LC analyte fractions) of metabolites. This instrument will be particularly useful for the identification and structural characterization of unknowns (ie. Metabolites that are not annotated in current NMR or MS databases of small molecules). The new system has been ordered from Bruker Inc. and should be delivered at MSU in June-July 2016.
During operation of the LC-SPE-NMR-MS, a tiny fraction of the liquid chromatography (LC) effluent (1-5%) will be directed to the MS system that detects and commands which fractions of the LC flow appear to contain interesting compounds; those will be captured on a fraction collection system for most of the LC sample stream, using a movable array of solid phase extraction (SPE) cartridges. The compounds trapped on the SPE cartridges will then be eluted following the LC run, and dried in NMR sample tubes. NMR solvents will be subsequently added and racks of tubes moved to the automatic sample loading system (autosampler) of the NMR instrument, where a variety of 1D and 2D NMR experiments will be used for small molecule identification and structure determination.